EVALUATION OF ANCHORAGE-DEPENDENT CELL PROPAGATION SYSTEMS FOR PRODUCTION OF HUMAN ACETYLCHOLINESTERASE BY RECOMBINANT 293 CELLS

Production of recombinant human acetylcholinesterase (AChE) by a high producer human embryonic kidney cell line (293) was evaluated by three main cell propagation systems; surface propagator, fixed-bed reactor and stirred microcarrier cultures. The recombinant cell line expresses AChE levels as high as 10-20 mg/l/day. System productivities in eithe r the surface propagator (multitray system), or in the fixed-bed react or (polyurethane macroporous sponges) were 4-8 mg AChE/l/day during a production period of 8 days. Similar productive rates, yet longer prod uction periods (up to 22 days), were obtained in microcarrier (MC) cul tures using either polystyrene beads (Biosilon); collagen-coated dextr an beads (Cytodex-3); or gelatin macroporous beads (Cultispher-G). Bes t results were obtained in an aggregate culture using cellulose beads charged with diethylaminoethyl (DEAE) groups, (Servacel), as carriers. In this culture, a system productivity of 6-10 mg/l/day was maintaine d for 28 days.