HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SEPARATION OF CHLORAMBUCIL AND ITS N-OXIDE PRODRUG

Authors
CHANDLER KJ MCCABE JB KIRKPATRICK DL
Citation
Kj. Chandler et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SEPARATION OF CHLORAMBUCIL AND ITS N-OXIDE PRODRUG, Journal of chromatography B. Biomedical applications, 652(2), 1994, pp. 195-202
Citations number
16
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
652
Issue
2
Year of publication
1994
Pages
195 - 202
Database
ISI
SICI code
Abstract
A reversed-phase high-performance liquid chromatographic method is des cribed to distinguish chlorambucil N-oxide from the parent chlorambuci l and quantitate both after separation from biological samples. The in fluence of solvent pH, alcohol, acid and ion-pairing agent on the sepa ration is described. The stability of chlorambucil and its N-oxide in buffers and alcohols, as well as stability during filtration is discus sed with potential application for metabolic studies.