Vc. Daniel et al., SIMPLIFIED ASSAY FOR THE QUANTIFICATION OF 2,3-DINOR-6-KETO-PROSTAGLANDIN-F(1-ALPHA) BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY, Journal of chromatography B. Biomedical applications, 653(2), 1994, pp. 117-122
Citations number
20
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
Endogenous prostacyclin production is best assessed by the measurement
of its excreted metabolites, of which a major one is 2,3-dinor-6-keto
prostaglandin F1alpha (2,3-dinor-6-keto-PGF1alpha). Gas chromatographi
c-mass spectrometric (GC-MS) assays have been developed for this compo
und but are cumbersome and time-consuming. We now report a modified as
say for the measurement of 2,3-dinor-6-keto-PGF1alpha employing GC-MS
in which sample preparation time is markedly shortened by replacing a
number of extraction steps with reversed-phase column extraction and b
y modifying derivatization procedures. Precision of the assay is +/- 5
% and the accuracy is 98%. The lower limit of detection in urine is ap
proximately 15 pg/mg creatinine. Normal urinary levels of this metabol
ite were found to be 141 +/- 54 pg/mg creatinine (mean +/- S.D.). Urin
ary excretion of 2,3-dinor-6-keto-PGF1alpha is markedly altered in sit
uations associated with abnormalities of prostacyclin generation when
quantified using this assay. Thus, this assay provides a sensitive and
accurate method to assess endogenous prostacyclin production and to f
urther explore the role of this compound in human health and disease.