SIMPLIFIED ASSAY FOR THE QUANTIFICATION OF 2,3-DINOR-6-KETO-PROSTAGLANDIN-F(1-ALPHA) BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

Citation
Vc. Daniel et al., SIMPLIFIED ASSAY FOR THE QUANTIFICATION OF 2,3-DINOR-6-KETO-PROSTAGLANDIN-F(1-ALPHA) BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY, Journal of chromatography B. Biomedical applications, 653(2), 1994, pp. 117-122
Citations number
20
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
653
Issue
2
Year of publication
1994
Pages
117 - 122
Database
ISI
SICI code
Abstract
Endogenous prostacyclin production is best assessed by the measurement of its excreted metabolites, of which a major one is 2,3-dinor-6-keto prostaglandin F1alpha (2,3-dinor-6-keto-PGF1alpha). Gas chromatographi c-mass spectrometric (GC-MS) assays have been developed for this compo und but are cumbersome and time-consuming. We now report a modified as say for the measurement of 2,3-dinor-6-keto-PGF1alpha employing GC-MS in which sample preparation time is markedly shortened by replacing a number of extraction steps with reversed-phase column extraction and b y modifying derivatization procedures. Precision of the assay is +/- 5 % and the accuracy is 98%. The lower limit of detection in urine is ap proximately 15 pg/mg creatinine. Normal urinary levels of this metabol ite were found to be 141 +/- 54 pg/mg creatinine (mean +/- S.D.). Urin ary excretion of 2,3-dinor-6-keto-PGF1alpha is markedly altered in sit uations associated with abnormalities of prostacyclin generation when quantified using this assay. Thus, this assay provides a sensitive and accurate method to assess endogenous prostacyclin production and to f urther explore the role of this compound in human health and disease.