DETERMINATION OF OXYTOCIN IN BIOLOGICAL SAMPLES BY ISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COULOMETRIC DETECTION USING C-18 SOLID-PHASE EXTRACTION AND POLYCLONAL ANTIBODY-BASED IMMUNOAFFINITY COLUMN PURIFICATION

Authors
KUKUCKA MA MISRA HP
Citation
Ma. Kukucka et Hp. Misra, DETERMINATION OF OXYTOCIN IN BIOLOGICAL SAMPLES BY ISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COULOMETRIC DETECTION USING C-18 SOLID-PHASE EXTRACTION AND POLYCLONAL ANTIBODY-BASED IMMUNOAFFINITY COLUMN PURIFICATION, Journal of chromatography B. Biomedical applications, 653(2), 1994, pp. 139-145
Citations number
17
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
653
Issue
2
Year of publication
1994
Pages
139 - 145
Database
ISI
SICI code
Abstract
A specific high-performance liquid chromatographic (HPLC) method is de scribed for the reliable quantitation of oxytocin using culture media supernatants. The procedure employs solid-phase extraction, antibody-b ased immunoaffinity purification and isocratic HPLC with dual channel coulometric detection (ED). The lower limit of detection for this cycl ic nonapeptide was 40 pg (40 fmol). Due to its relative simplicity, sp ecificity and precision, the HPLC-ED of oxytocin is an accurate and at tractive alternative to many existing quantitative methods.