BOOPHILINE, AN ANTIMICROBIAL STEROL AMIDE FROM THE CATTLE TICK BOOPHILUS-MICROPLUS

Boophiline (1), a new sterol amide was isolated from the cattle tick B oophilus microplus (Ixodidae). The structure was assigned as N-[3-(sul fooxy)-25 xi-cholest-5-en-26-oyl]-L-isoleucine by detailed 2D NMR inve stigations in conjunction with FAB mass spectrometry and acidic hydrol yses. Complete assignment of the diastereotopic methylene protons of t he ring system could be deduced From the NMR data. In agar dilution as says, 1 exhibited antifungal properties against Cladosporium cucumerin um and antibacterial activity against Bacillus subtilis and Escherichi a coli.