A RETRO-INVERSO PEPTIDE CORRESPONDING TO THE GH LOOP OF FOOT-AND-MOUTH-DISEASE VIRUS ELICITS HIGH-LEVELS OF LONG-LASTING PROTECTIVE NEUTRALIZING ANTIBODIES

Citation
Jp. Briand et al., A RETRO-INVERSO PEPTIDE CORRESPONDING TO THE GH LOOP OF FOOT-AND-MOUTH-DISEASE VIRUS ELICITS HIGH-LEVELS OF LONG-LASTING PROTECTIVE NEUTRALIZING ANTIBODIES, Proceedings of the National Academy of Sciences of the United Statesof America, 94(23), 1997, pp. 12545-12550
Citations number
30
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
94
Issue
23
Year of publication
1997
Pages
12545 - 12550
Database
ISI
SICI code
0027-8424(1997)94:23<12545:ARPCTT>2.0.ZU;2-F
Abstract
Peptides corresponding to the immunodominant loop located at residues 135-158 on capsid protein VP1 of foot-and-mouth disease virus (FMDV) g enerally elicit high levels of anti-peptide and virus-neutralizing ant ibodies. In some instances, however, the level of neutralizing antibod ies is low or even negligible, even though the level of anti-peptide a ntibodies is high, We have shown previously that the antigenic activit y of peptide 141-159 of VP1 of a variant of serotype A can be mimicked by a retro-inverso (all-D retro or retroenantio) peptide analogue, Th is retro-inverso analogue induced greater and longer-lasting antibody titers than did the corresponding L-peptide, We now show that a single inoculation of the retro-inverso analogue elicits high levels of neut ralizing antibodies that persist longer than those induced against the corresponding L-peptide and confer substantial protection in guinea p igs challenged with the cognate virus, In view of the high stability t o proteases of retro-inverso peptide analogues and their enhanced immu nogenicity, these results have practical relevance in designing potent ial peptide vaccines.