H. Kiyosawa et al., COMPATIBLE DICTYOSTELIUM-MUCOROIDES NUCLEAR PLASMIDS DMP1 AND DMP2 BOTH BELONG TO THE DDP1 PLASMID FAMILY, Plasmid, 31(2), 1994, pp. 121-130
Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurrin
g compatible members of the Dictyostelium Ddp1 plasmid family found in
the same wild isolate strain. The nucleotide sequences of Dmp1 (5983
bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading
frames (ORFs) similar to the G1 and G5 ORFs of the Dictyostelium disc
oideum plasmid Ddp1. The predicted protein product of the Ddp1 G1 ORF
is 49% similar to that of the Dmp1 G1-like ORF and 52% similar to that
of the Dmp2 G1-like ORF. For the G5 and G5-like ORFs the correspondin
g values are 47 and 43%, respectively. The G1 and G5 ORFs of Ddp1 are
transcribed during both vegetative growth and development of the asexu
al fruiting body. The G1-like ORF of Dmp2 is expressed in vegetative a
nd developing cells, while that of Dmp1 appears to be expressed mostly
in developing cells. The G5-like ORFs of Dmp1 and Dmp2 are expressed
in both vegetative and developing cells. Dmp1 and Dmp2 differ from Ddp
1 in (1) lacking homologs for the Ddp1 G2/G3/D4, G4/D5, D1/D3, and D2
ORFs; (2) containing multiple copies of a 173 bp direct repeat, and (3
) having a different orientation of the G1 ORF relative to the G5 ORF.
These findings suggest that the basic replicon unit of the Ddp1 plasm
id family is composed of an origin of replication coupled to G1-like a
nd G5-like genes. The additional ORFs and direct repeat elements in Dd
p1, Dmp1, and Dmp2 may provide accessory functions beneficial to plasm
id maintenance. Shuttle vectors based on Dmp1 or Dmp2 replicate in D.
discoideum transformants. (C) 1994 Academic Press, Inc.