DETERMINATION OF FREE-CHOLESTEROL FROM DRIED EGG-YOLK BY ONLINE COUPLING OF SUPERCRITICAL-FLUID EXTRACTION WITH SOLID-PHASE EXTRACTION

Authors
BOSELLI E CABONI MF LERCKER G
Citation
E. Boselli et al., DETERMINATION OF FREE-CHOLESTEROL FROM DRIED EGG-YOLK BY ONLINE COUPLING OF SUPERCRITICAL-FLUID EXTRACTION WITH SOLID-PHASE EXTRACTION, ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 205(5), 1997, pp. 356-359
Citations number
20
Categorie Soggetti
Food Science & Tenology
Journal title
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY → ACNP
ISSN journal
14314630
Volume
205
Issue
5
Year of publication
1997
Pages
356 - 359
Database
ISI
SICI code
1431-4630(1997)205:5<356:DOFFDE>2.0.ZU;2-2
Abstract
A classic solvent extraction and a supercritical CO2 extraction (SFE) method for the determination of free cholesterol in dried egg yolk wer e compared. After lipid extraction, a solid-phase extraction (SPE) was performed to eliminate the non-polar lipids and to purify cholesterol . Free cholesterol was then determined by capillary gas chromatography . The supercritical extractor enabled on line SPE after SFE, resulting in a shorter extraction time compared with that of the classic method . Improved repeatability and a smaller amount of solvent required are the main advantages of the method proposed here compared with the clas sic solvent extraction method.