G. Began et al., ENRICHMENT OF PHOSPHATIDYL CHOLINE - ALCOHOL FRACTIONATION OF CARBON-DIOXIDE DE-OILED LECITHIN FROM SOYBEAN (GLYCINE-MAX), ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 205(5), 1997, pp. 360-363
Crude soylecithin was extracted with carbon dioxide at pressures of 12
0, 200, 240, 280 and 320 bar (12, 20, 24, 28 and 32 MPa, respectively)
and at a temperature of 40 degrees C to obtain de-oiled lecithin. The
amount of phospholipids (acetone insoluble) increased with increasing
pressure. The carbon-dioxide-extracted lecithin (CDEL) was fractionat
ed with alcohol into alcohol-soluble and alcohol-insoluble fractions.
Depending on the pressure, the alcohol-soluble fraction of CDEL was fo
und to contain up to 70.0% phosphatidyl choline as against 21.1% prese
nt in crude lecithin. The alcohol-insoluble fraction of CDEL was found
to contain up to 37.1% phosphatidyl inositol as against 12.3% in crud
e lecithin. The chemical parameters of CDEL and the alcohol-soluble an
d alcohol-insoluble fractions were evaluated.