D. Mingeot et Jm. Jacquemin, A WHEAT CDNA CODING FOR A THAUMATIN-LIKE PROTEIN REVEALS A HIGH-LEVELOF RFLP IN WHEAT, Theoretical and Applied Genetics, 95(5-6), 1997, pp. 822-827
A cDNA clone that reveals a high level of polymorphism between wheat v
arieties was isolated from a wheat cDNA library. When hybridized to Dr
aI-, EcoRV- and HindIII digested DNA this clone, gbx3832, enables us t
o distinguish 42 different patterns among 48 varieties: 37 varieties a
re clearly identified, the remaining 11 are divided into five groups.
Base-sequence analysis of the clone reveals 72-74 % sequence identity
to mRNAs encoding thaumatin-like proteins from different cereals.