ONTOGENIC DEVELOPMENT OF ANASTREPHA-FRATERCULUS (DIPTERA, TEPHRITIDAE) - ISOENZYME PATTERNS OF ISOCITRATE AND ALCOHOL DEHYDROGENASES

Anastrepha fraterculus was analysed by electrophoresis during the whol e development period. Two Isocitrate dehydrogenase (IDH) loci were fou nd, one of them expressed from first-stage to 100-day-old adults with varied intensity of staining; the other one was detected only from thi rd-stage larvae until 216-hr pupae. Three presumptive Alcohol dehydrog enase (ADH) loci and 14 electromorphs were detected. Isopropanol was t he best ADH substrate for adults and both isopropanol and ethanol were similar as good substrates for third-stage larvae. The ADH-1 locus pr esented two to four electromorphs and its product was better visualize d in starch gel; ADH-2 showed three electromorphs and ADH-3 seven elec tromorphs; polyacrylamide gel was better for the latter isoenzymes. Th e three loci were activated in third-stage larvae, pupal stage, and ad ult period; ADH-1 and ADH-3 were present in the second-stage larvae, a nd no ADH activity was detected during the embryonic period and first stage larvae. Males and females (more than 30 days old) showed higher ADH activity; the ADH of females was more active in 100-day-old flies. A specific locus for Octanol dehydrogenase was not detected. Third-st age larvae reared in papaya, guava, and banana show identical ADH patt erns. No activity of this enzyme was detected when larvae were grown i n kiwi. (C) 1997 Elsevier Science Inc.