INDUCTION OF SERUM AMYLOID-A (SAA) GENE BY SAA-ACTIVATING SEQUENCE-BINDING FACTOR (SAF) IN MONOCYTE MACROPHAGE CELLS - EVIDENCE FOR A FUNCTIONAL SYNERGY BETWEEN SAF AND SP1/
Bk. Ray et A. Ray, INDUCTION OF SERUM AMYLOID-A (SAA) GENE BY SAA-ACTIVATING SEQUENCE-BINDING FACTOR (SAF) IN MONOCYTE MACROPHAGE CELLS - EVIDENCE FOR A FUNCTIONAL SYNERGY BETWEEN SAF AND SP1/, The Journal of biological chemistry, 272(46), 1997, pp. 28948-28953
Serum amyloid A (SAA) is a plasma protein that is associated with many
inflammatory diseases including amyloidosis, arthritis, and atheroscl
erosis. SAA level is significantly increased during inflammatory condi
tion, and such abnormal expression of this protein is linked to the pa
thogenesis of the above-mentioned diseases, A promoter element, design
ated as SAA-activating sequence (SAS), located between -280 and -226 h
as been implicated in the induction mechanism and a nuclear factor, SA
S-binding factor (SAF), has been shown to bind to this region, In this
report, using a cloned SAF gene in transient transfection assay. we p
rovide evidence that SAF potentiates SAA gene expression through SAS e
lement. Furthermore, we show that during lipopolysaccharide-mediated i
nduction of SAF, het eromeric complex with transcription factor Sp1 is
formed, Transfection assays using both transcription factor genes hav
e demonstrated that SAF-Sp1 heteromer is a highly potent transactivato
r of SAA expression.