DNA-SEQUENCE, CHROMOSOMAL LOCALIZATION, AND TISSUE EXPRESSION OF THE MOUSE PROTEASOME SUBUNIT LMP10 (PSMB10) GENE

Proteasomes are nonlysosomal multicatalytic proteases involved in anti gen processing, Three of the 10 mammalian proteasome p subunits (LMP2, LMP7, and LMP10) are induced by IFN-gamma. Two of these (LMP2 and LMP 7) are encoded in the major histocompatibility complex of both human ( chromosome 6) and mouse (chromosome 17). However, the human homologue of Lmp10, MECL1, is found on chromosome 16, Here we show that in mice, Lmp10 is a single-copy gene localized to chromosome 8, in a region of conserved synteny with human chromosome 16. Sequencing of a 129/SvJ s train genomic clone revealed that the gene has eight exons spanning 2. 3 kb, Characterization of a full-length mouse cDNA clone indicates tha t Lmp10 encodes a protein of 273 amino acids with a calculated molecul ar weight of 29 kDa and an isoelectric point of 6.86. Northern analysi s of Lmp2, Lmp7, and Lmp10 showed expression in heart, liver, thymus, lung, and spleen, but not in brain, kidney, skeletal muscle, or testis . (C) 1997 Academic Press.