GAS-CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF VOLATILE FLUOROETHER ANESTHETICS BY DERIVATIZED CYCLODEXTRINS .2. PREPARATIVE-SCALE SEPARATIONS FOR ISOFLURANE
Du. Staerk et al., GAS-CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF VOLATILE FLUOROETHER ANESTHETICS BY DERIVATIZED CYCLODEXTRINS .2. PREPARATIVE-SCALE SEPARATIONS FOR ISOFLURANE, Journal of chromatography, 663(1), 1994, pp. 79-85
Preparative-scale gas chromatographic separation of the enantiomers of
isoflurane, a volatile anesthetic, has been achieved with 2 m x 10 mm
I.D. columns, packed with 80-100 mesh Chromosorb WAW, coated with up
to 23% (w/w) trifluoroacetyl gamma-cyclodextrin as stationary phase. T
he type and particle size of the support, the concentration of the sta
tionary phase and the separation conditions were varied to improve pro
duct purity and the production rate under overloaded elution mode cond
itions. Using a sampling interface located between the exit of the pre
parative column and the fraction collector, effluent samples were dire
cted onto a short, high-efficiency analytical capillary column every 3
0 s to achieve on-line enantiomeric analysis of the eluting bands, whi
ch allowed the calculation of purity, recovery and production rate for
the separations.