HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ATRACTYLOSIDE AND CARBOXYATRACTYLOSIDE FROM ATRACTYLIS-GUMMIFERA L

A reversed-phase high-performance liquid chromatographic method is des cribed for the identification and determination of two toxic compounds , atractyloside and carboxyatractyloside, in a 60% methanolic extract of Atractylis gummifera L. roots. Separation was achieved in 55 min wi th a water-acetonitile gradient in the presence of trifluoroacetic aci d. Detection was effected with a light-scattering detector. The detect ion limit was determined and was ca. 9 mu g of atractyloside standard injected. The method of quantification was validated.