A COLORIMETRIC FORMALDEHYDE ASSAY

Authors
Citation
Ka. Taylor, A COLORIMETRIC FORMALDEHYDE ASSAY, Applied biochemistry and biotechnology, 68(1-2), 1997, pp. 81-93
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
ISSN journal
02732289
Volume
68
Issue
1-2
Year of publication
1997
Pages
81 - 93
Database
ISI
SICI code
0273-2289(1997)68:1-2<81:ACFA>2.0.ZU;2-G
Abstract
A room-temperature assay of formaldehyde is described. The assay uses few reagents and is colorimetric, read at a wavelength of 649 nm. Tryp tophan and tryptamine were noted as interfering with the assay, probab ly by binding with the formaldehyde. High levels of sugar show smaller effects on final absorbance. Glyceraldehyde also reacts in the assay, but six other aldehyde compounds do not, although they do reduce the absorbance of added formaldehyde.