CHARACTERIZATION OF NOVEL NEOPULLULANASE FROM BACILLUS-POLYMYXA

Authors
YEBRA MJ ARROYO J SANZ P PRIETO JA
Citation
Mj. Yebra et al., CHARACTERIZATION OF NOVEL NEOPULLULANASE FROM BACILLUS-POLYMYXA, Applied biochemistry and biotechnology, 68(1-2), 1997, pp. 113-120
Citations number
21
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
68
Issue
1-2
Year of publication
1997
Pages
113 - 120
Database
ISI
SICI code
0273-2289(1997)68:1-2<113:CONNFB>2.0.ZU;2-K
Abstract
Bacillus polymyxa CECT 155 produces an extracellular neopullulanase ac tivity that degrades pullulan to panose. This activity was stimulated by the presence of pullulan in the culture, and repressed by glucose. The apparent mot wt determined for the enzyme was 58 kDa. The optimum pH and temperature for neopullulanase activity were pH 6.0 and 50 degr ees C, respectively. The enzyme was stable in a pH range of 4.0-8.0, a nd temperatures up to 60 degrees C. These properties make it suitable for the saccharification processes in the starch industries.