IMIDAZOLE - A NEW LIGAND FOR METAL AFFINITY PRECIPITATION - PRECIPITATION OF KUNITZ SOYBEAN TRYPSIN-INHIBITOR USING CU(II)-LOADED COPOLYMERS OF 1-VINYLIMIDAZOLE WITH N-VINYLCAPROLACTAM OR N-ISOPROPYLACRYLAMIDE
Iy. Galaev et al., IMIDAZOLE - A NEW LIGAND FOR METAL AFFINITY PRECIPITATION - PRECIPITATION OF KUNITZ SOYBEAN TRYPSIN-INHIBITOR USING CU(II)-LOADED COPOLYMERS OF 1-VINYLIMIDAZOLE WITH N-VINYLCAPROLACTAM OR N-ISOPROPYLACRYLAMIDE, Applied biochemistry and biotechnology, 68(1-2), 1997, pp. 121-133
Kunitz soybean trypsin inhibitor (STI) was specifically coprecipitated
during precipitation of Cu(II)-loaded copolymers induced by increase
in temperature and ionic strength. The copolymers used consisted of 1-
vinylimidazole and N-vinylcaprolactam or N-isopropylacrylamide. The el
ution of STI was achieved by solubilization of the STI-Cu(II)-polymer
complex in the presence of an excess of the competing ligand, imidazol
e, and a subsequent precipitation of the polymer with STI remaining fr
ee in solution in a purified form as judged by Sodium dodecylsulfate-p
olyacrylamide gel electrophoresis (SDS-PAGE). To the best of our knowl
edge this is the first reported successful metal affinity precipitatio
n of protein in a heterobifunctional format.