MECHANISM OF DETHIOBIOTIN SYNTHETASE - CHARACTERIZATION OF THE 8-AMINOCARBAMATE OF (7R,8S)-7,8 DIAMINONONANOATE AS AN ENZYME-BOUND INTERMEDIATE

Authors
BAXTER RL RAMSEY AJ MCIVER LA BAXTER HC
Citation
Rl. Baxter et al., MECHANISM OF DETHIOBIOTIN SYNTHETASE - CHARACTERIZATION OF THE 8-AMINOCARBAMATE OF (7R,8S)-7,8 DIAMINONONANOATE AS AN ENZYME-BOUND INTERMEDIATE, Journal of the Chemical Society, Chemical Communications, (5), 1994, pp. 559-560
Citations number
10
Categorie Soggetti
Chemistry
Journal title
Journal of the Chemical Society, Chemical Communications → ACNP
ISSN journal
00224936
Issue
5
Year of publication
1994
Pages
559 - 560
Database
ISI
SICI code
0022-4936(1994):5<559:MODS-C>2.0.ZU;2-8
Abstract
Enzymatically catalysed formation of the ureido ring of dethiobiotin 2 from (7R, 8S)-7,8-diaminononanoate 3 by purified E. coli dethiobiotin synthetase involves the formal hydrolysis of one equivalent of ATP, a combination of substrate loading and trapping experiments show that t he carbamate 5 is an intermediate in the synthesis of dethiobiotin fro m 3.