PURIFICATION AND CHARACTERIZATION OF XYLANASES FROM TRICHODERMA

Xylanase preparations free of cellulase contamination are often requir ed for applications in the pulp and paper industry. Xylanases from Tri choderma have been purified and characterized in an effort to better u nderstand the behavior of the various components of the xylanolytic sy stem in order to optimize their effect However purification and charac terization of xylanases from Trichoderma species are still incomplete. This review organizes current techniques for xylanase purification an d examines the properties of xylannses that have been previously chara cterized Problems with assays, lyophilizing, gel permeation behavior; gel electrophoresis and developing are also noted. From this review, a strategy for the isolation of an endoxylanase from a commercial prepa ration of T. longibrachiatum is developed. Published by Elsevier Scien ce Ltd.