HEME PEROXIDASE-ACTIVITY MEASURED IN SINGLE MOSQUITOS IDENTIFIES INDIVIDUALS EXPRESSING AN ELEVATED OXIDASE FOR INSECTICIDE RESISTANCE

Authors
BROGDON WG MCALLISTER JC VULULE J
Citation
Wg. Brogdon et al., HEME PEROXIDASE-ACTIVITY MEASURED IN SINGLE MOSQUITOS IDENTIFIES INDIVIDUALS EXPRESSING AN ELEVATED OXIDASE FOR INSECTICIDE RESISTANCE, Journal of the American Mosquito Control Association, 13(3), 1997, pp. 233-237
Citations number
25
Categorie Soggetti
Entomology
Journal title
Journal of the American Mosquito Control Association → ACNP
ISSN journal
8756971X
Volume
13
Issue
3
Year of publication
1997
Pages
233 - 237
Database
ISI
SICI code
8756-971X(1997)13:3<233:HPMISM>2.0.ZU;2-7
Abstract
Optimum conditions are described for a simple, rapid, microplate-based assay that indirectly measures the differences in oxidase levels betw een individual susceptible, resistant, or induced mosquitoes. A small proportion (0.01-0.1) of a single mosquito is used, allowing multiple replicates of the oxidase assay. Cytochrome C is used as a positive co ntrol. The levels of oxidase found in sample populations of pyrethroid -susceptible, pyrethroid-resistant, and phenobarbital-induced Anophele s albimanus mosquitoes are characterized with the assay.