GENOMIC (5'UTR) AND SEROLOGICAL DIFFERENCES AMONG GERMAN BVDV FIELD ISOLATES

Authors
WOLFMEYER A WOLF G BEER M STRUBE W HEHNEN HR SCHMEER N KAADEN OR
Citation
A. Wolfmeyer et al., GENOMIC (5'UTR) AND SEROLOGICAL DIFFERENCES AMONG GERMAN BVDV FIELD ISOLATES, Archives of virology, 142(10), 1997, pp. 2049-2057
Citations number
29
Categorie Soggetti
Virology
Journal title
Archives of virology → ACNP
ISSN journal
03048608
Volume
142
Issue
10
Year of publication
1997
Pages
2049 - 2057
Database
ISI
SICI code
0304-8608(1997)142:10<2049:G(ASDA>2.0.ZU;2-S
Abstract
Isolates of bovine viral diarrhoea virus (BVDV) collected in Germany w ere examined for their genomic heterogeneity in sequences from the 5'u ntranslated region (UTR) of the viral genome. Polymerase chain reactio n (PCR) tests based on the 5'UTR and the region coding for the NS2-3/4 A polypeptide were used to differentiate between BVDV I and BVDV II ge notypes. Eleven out of 96 BVDV-isolates were identified as BVDV II. Vi rus neutralization tests with BVDV I-or II-specific antisera raised in cattle were done. The mean titers were reduced by 7.2-fold (BVDV I-an tiserum versus type II-isolates) or 35-fold (BVDV II-antiserum versus type I-isolates) when using the respective heterologous virus.