Di. Oppenheimer et Le. Volkman, EVIDENCE FOR ROLLING CIRCLE REPLICATION OF AUTOGRAPHA-CALIFORNICA M-NUCLEOPOLYHEDROVIRUS GENOMIC DNA, Archives of virology, 142(10), 1997, pp. 2107-2113
Antographa californica M nucleopolyhedrovirus (AcMNPV) is a large ds D
NA virus restricted to larval lepidopteran insect hosts. Using field i
nversion gel electrophoresis and digestion with a restriction enzyme w
hich cuts the AcMNPV genome once, we detected multiple unit-length gen
ome fragments from replicating viral DNA. Our data suggest that AcMNPV
replicates in a head-to-tail manner via rolling circle replication.