PTERIDINE NUCLEOSIDES NEW - NEW VERSATILE BUILDING-BLOCKS IN OLIGONUCLEOTIDE SYNTHESIS

Authors
CHARUBALA R MAURINSH J ROSLER A MELGUIZO M JUNGMANN O GOTTLIEB M LEHBAUER J HAWKINS M PFLEIDERER W
Citation
R. Charubala et al., PTERIDINE NUCLEOSIDES NEW - NEW VERSATILE BUILDING-BLOCKS IN OLIGONUCLEOTIDE SYNTHESIS, Nucleosides & nucleotides, 16(7-9), 1997, pp. 1369-1378
Citations number
16
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
16
Issue
7-9
Year of publication
1997
Pages
1369 - 1378
Database
ISI
SICI code
0732-8311(1997)16:7-9<1369:PNN-NV>2.0.ZU;2-J
Abstract
Chemical syntheses of 1-(2-deoxy-beta-D-ribofuranosyl)lumazines and is opterins as well as eoxy-beta-D-ribofuranosyl)-4-amino-7(8H)pteridones and -isoxanthopterins have been developed to make the structural anal ogs of the naturally occurring 2'-deoxyribonucleosides in the pteridin e series available. The corresponding phosphoramidites have been used in machine-aided solid-support syntheses leading to new types of fluor escence labeled oligonucleotides. The effects of the various fluoropho rs on duplex formation and as labels for enzyme reactions is demonstra ted.