OLIGONUCLEOTIDES CONTAINING N-7-CYANOBORANE-2'-DEOXYGUANOSINE

For synthesis of N-7-cyanoborane-containing oligonucleotides, the 5'-D MT protecting group is not a suitable precursor because the boronated nucleoside is incompatible with DMT cations released during deprotecti on of the oligonucleotide. As an alternative to DMT, we have investiga ted use of the 5'-Fmoc protecting group. We found that the cyanoborane group is stable during synthesis and deprotection conditions used wit h Fmoc derivatives.