IMPROVED IMPURITY PROFILE OF PHOSPHOROTHIOATE OLIGONUCLEOTIDES THROUGH THE USE OF DIMERIC PHOSPHORAMIDITE SYNTHONS

Authors
KROTZ AH KLOPCHIN P COLE DL RAVIKUMAR VT
Citation
Ah. Krotz et al., IMPROVED IMPURITY PROFILE OF PHOSPHOROTHIOATE OLIGONUCLEOTIDES THROUGH THE USE OF DIMERIC PHOSPHORAMIDITE SYNTHONS, Nucleosides & nucleotides, 16(7-9), 1997, pp. 1637-1640
Citations number
20
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
16
Issue
7-9
Year of publication
1997
Pages
1637 - 1640
Database
ISI
SICI code
0732-8311(1997)16:7-9<1637:IIPOPO>2.0.ZU;2-S
Abstract
Phosphorothioate oligonucleotides synthesized through assembly of dime ric phosphoramidite synthons show a significantly improved impurity pr ofile compared to oligomers synthesized through coupling of standard m onomer phosphoramidites. A greater than 70% reduction of the (n-1)-mer population and a ca. 50% reduction of phosphodiester linkages has bee n achieved.