STABILITY OF STEREOREGULAR OLIGO(NUCLEOSIDE PHOSPHOROTHIOATE)S IN HUMAN-CELLS - DIASTEREOSELECTIVITY OF CELLULAR 3'-EXONUCLEASE

Authors
KOZIOLKIEWICZ M GENDASZEWSKA E MASZEWSKA M
Citation
M. Koziolkiewicz et al., STABILITY OF STEREOREGULAR OLIGO(NUCLEOSIDE PHOSPHOROTHIOATE)S IN HUMAN-CELLS - DIASTEREOSELECTIVITY OF CELLULAR 3'-EXONUCLEASE, Nucleosides & nucleotides, 16(7-9), 1997, pp. 1677-1682
Citations number
8
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
16
Issue
7-9
Year of publication
1997
Pages
1677 - 1682
Database
ISI
SICI code
0732-8311(1997)16:7-9<1677:SOSOPI>2.0.ZU;2-3
Abstract
Stability of oligo(nucleoside phosphorothioate)s (PS-oligos) in HUVEC (human umbilical vein endothelial cells) has been studied. Cytosolic f raction of HUVEC possesses 3'-exonucleolytic activity which is respons ible for degradation of natural oligomers and PS-oligos. The enzyme is R-P-specific, i.e. it cleaves internucleotide phosphorothioate functi on of R-P- and not S-P-configuration at phosphorus atom.