F. Lopezgarcia et al., DIACYLGLYCEROL, PHOSPHATIDYLSERINE AND CA2- A PHASE-BEHAVIOR STUDY( ), Biochimica et biophysica acta. Biomembranes, 1190(2), 1994, pp. 264-272
The interaction of 1,2-dipalmitoylglycerol (1,2-DPG) with dipalmitoylp
hosphatidylserine (DPPS) has been studied in the presence and in the a
bsence of Ca2+ by using differential scanning calorimetry (DSC) and P-
31-nuclear magnetic resonance (P-31-NMR). In the absence of Ca2+, DSC
showed that 1,2-DPG increased the phase transition of DPPS, effect alr
eady noticed at very low 1,2-DPG concentrations, whereas lipid immisci
bilities were detected at concentrations of 1,2-DPG higher than about
30 mol%. P-31-NMR indicated that lamellar phases were always present a
t concentrations of 1,2-DPG lower than about 35 mol%, but at higher co
ncentrations non-lamellar phases may be present in the fluid phase. As
observed by DSC, the apparent pK(a) of the carboxyl group of DPPS was
increased slightly in the presence of 1,2-DPG. In the presence of Ca2
+, the effect of 1,2-DPG was to further increase the temperature of th
e onset of the phase transition, indicating an stabilization of the mo
st rigid phase in the DPPS/1,2-DPG/Ca2+ samples. Even concentrations o
f 1,2-DPG as low as 1 mol% of the total lipid already produced a notic
eable effect. Moreover, lipid immiscibilities were apparent at concent
rations of 1,2-DPG higher than 20 mol%. Furthermore, the transition of
the DPPS/Ca2+ complex observed by DSC at 155-degrees-C was perturbed
by the presence of 1,2-DPG, indicating a change in the structure of th
e crystalline complex. Interestingly, the effect of non-saturating Ca2
+ concentrations on the DPPS phase transition was enhanced by the pres
ence of 1,2-DPG. The effect reported here may be significant for a num
ber of situations where Ca2+, phosphatidylserine and diacylglycerols a
re involved, such as fusion of membranes, where diacylglycerol may fac
ilitate Ca2+-induced fusion, or the activation of enzymes such as prot
ein kinase C and phospholipases.