AN ELECTROPHORETIC MOLECULAR KARYOTYPE OF A CLINICAL ISOLATE OF ASPERGILLUS-FUMIGATUS AND LOCALIZATION OF THE MDR-LIKE GENES AFUMDR1 AND AFUMDR2

Authors
TOBIN MB PEERY RB SKATRUD PL
Citation
Mb. Tobin et al., AN ELECTROPHORETIC MOLECULAR KARYOTYPE OF A CLINICAL ISOLATE OF ASPERGILLUS-FUMIGATUS AND LOCALIZATION OF THE MDR-LIKE GENES AFUMDR1 AND AFUMDR2, Diagnostic microbiology and infectious disease, 29(2), 1997, pp. 67-71
Citations number
26
Categorie Soggetti
Microbiology,"Infectious Diseases
Journal title
Diagnostic microbiology and infectious disease → ACNP
ISSN journal
07328893
Volume
29
Issue
2
Year of publication
1997
Pages
67 - 71
Database
ISI
SICI code
0732-8893(1997)29:2<67:AEMKOA>2.0.ZU;2-Y
Abstract
The molecular karyotype of a clinical isolate of Asperigillus fumigatu s (10AF/86/10) was determined by contour-clamped homogeneous electric field gel electrophoresis. Five chromosomal bands were resolved by thi s method. The resolved chromosomes ranged in size from 1.7 to 4.8 Mb, and together constituted a total genomic size of at least 15.8 Mb. Sou thern analysis of the separated chromosomes located the position of tw o MDR-like genes, AfuMDR1 and AfuMDR2, on chromosomes III and IV, resp ectively. The methods described herein may enable the application of m olecular karyotyping of A. fumigatus in epidemiologic surveillance stu dies. (C) 1997 Elsevier Science Inc.