NOSOCOMIAL ENTEROCOCCAL BLOOD-STREAM INFECTIONS IN THE SCOPE PROGRAM - ANTIMICROBIAL RESISTANCE, SPECIES OCCURRENCE, MOLECULAR TESTING RESULTS, AND LABORATORY TESTING ACCURACY
Rn. Jones et al., NOSOCOMIAL ENTEROCOCCAL BLOOD-STREAM INFECTIONS IN THE SCOPE PROGRAM - ANTIMICROBIAL RESISTANCE, SPECIES OCCURRENCE, MOLECULAR TESTING RESULTS, AND LABORATORY TESTING ACCURACY, Diagnostic microbiology and infectious disease, 29(2), 1997, pp. 95-102
Characteristics of nosocomial enterococcal blood stream infection (NEB
SI) isolates obtained from patients at 41 U.S. hospitals participating
in the SCOPE Program were studied. Isolates from 480 episodes of NEBS
I were characterized according to species and antimicrobial susceptibi
lity profile. Selected isolates were also identified to species and va
ncomycin resistance genotype using polymerase chain reaction based met
hods. Polymerase chain reaction genotyping and ribotyping were used as
genetic markers for molecular epidemiologic typing. Enterococci were
the third most common cause of nosocomial blood stream infection in th
is study, accounting for 11.7% of all isolates reported. Enterococcus
faecalis was the most common species (59.6%), followed by E. faecium (
19.4%). Species identification errors involving E. faecium, E. durans,
E. avium, and E. raffinosus were observed. Vancomycin resistance was
observed in 36.4% of all participating medical centers and varied from
11.1% of medical centers in the Northwest to 60.9% of medical centers
in the Southwest. Vancomycin-resistant enterococci accounted for 20.6
% of NEBSI in the Northeast, 11.4% in the Southeast, 11.1% in the Sout
hwest, and 9.5% in the Northwest regions. VanA genotypes predominated
in the Northeast and Southwest, whereas vanA and vanB genotypes were e
qually prevalent in the Northwest and Southeast. Molecular typing stud
ies identified strains that were unique to individual hospitals as wel
l as strins that were prevalent in several different hospitals. NEBSI
with vancomycin-resistant enterococci continues to escalate among hosp
italized patients in all geographic areas of the USA. (C) 1997 Elsevie
r Science Inc.