DETERMINATION OF PUERARIN, DAIDZEIN, PAEONIFLORIN, CINNAMIC ACID, GLYCYRRHIZIN, EPHEDRINE, AND [6]-GINGEROL IN GE-GEN-TANG BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

This paper presents a micellar electrokinetic chromatography (MEKC) me thod with diode-array detection to analyze for seven major components in the traditional Chinese medicinal preparation of Ge-gen-tang. Those analytes were successfully separated within 9 min using a pH 9.0 bera te buffer containing 20 mM Sodium dodecyl sulfate at 30 degrees C. Exp erimental results indicated that the relative standard deviations of t he analytes' migration times were <0.43% and the correlation coefficie nts of the analytes' linear calibration graphs exceeded 0.996. The qua ntities of the seven components in four different Ge-gen-tang samples were determined by the MEKC method coupled with a relatively simple ex traction method. (C) 1997 Elsevier Science B.V.