DIFFERENTIAL-PULSE POLAROGRAPHIC-DETERMINATION OF CEFTAZIDIME IN URINE SAMPLES WITH AND WITHOUT PRIOR EXTRACTION

Ceftazidime shows two main polarographic reduction peaks at pH 4.0, th at at -0.45 V owing to reduction of the C=N bond in the methylethoxyim ino group and that at -1.00 V owing to the reductive elimination of py ridine: the first peak is particularly suitable for the determination of ceftazidime. Ceftazidime can also be determined indirectly using th e tensammetric peak at -0.60 V (in Britton-Robinson buffer pH 9.5) of pyridine liberated on hydrolysis. Ceftazidime can be determined in uri ne using the direct method only after Cls solid phase extraction, but it can be determined directly in the urine by hydrolysing it and using the pyridine peak. (C) 1997 Elsevier Science B.V.