An amperometric flow cell was developed for continuous monitoring of l
actate in the subcutaneous microdialysate from a rat. The lactate meas
uring flow cell is based on a platinum-Ag/AgCl electrode covered with
a lactate oxidase containing sandwich membrane. The functional stabili
ty of the lactate probe was more than one-week and the results from th
e enzyme sensor measurement was confirmed by off-line measurements wit
h Liquid chromatography. (C) 1997 Elsevier Science B.V.