DETERMINATION OF DNA CONTENT IN BREAST-TU MOR CELL BY HADAMARD-TRANSFORM MICROSCOPIC IMAGE-ANALYSIS SYSTEM

In this study by means of acridine organge(AO) staining cell and Hadam ard transform microscopic image analysis system, some factors that aff ect fluorescence intensity of cellular AO-DNA DNA complex and accuracy of analytical results of human breast turner fell nuclear DNA content were investigated. The results show that: (1)ethanol is the best fixa tive for cell, and aldehydes have significant negative influence on ce ll fluorescence; (2)cells emit stronger fluorescence when AO contains Triton X-100; (3)the optimum concentration of AO is 50 mu g/mL; (4)whe n tumor cell nuclear UNA content is analyzed, satisfactory results can be obtained with either external control method (lymphocyte in blood smear or lymph node specimen smear is used as standard diploid cell) o r internal control method (normal epithelial cell in the same tumor sp ecimen is used as standard diploid cell), but the latter is preferable ; (5)reliable results carl be obtained when no less than sixty tumor c ells are measured at random for each case.