PURIFIED HRPA OF PSEUDOMONAS-SYRINGAE PV. TOMATO DC3000 REASSEMBLES INTO PILI

Pseudomonas syringae pv. tomato DC3000 produces Hrp pili under inducin g in vitro conditions. A preparation of partially purified extracellul ar filaments contains HrpA, flagellin and some minor contaminants. Hrp A was separated from the major contaminant, the flagellin, by gel filt ration to a fraction containing HrpA as well as its three N-terminally truncated forms, These were further separated by two steps of reverse d phase chromatography. HrpA and its degradation products were each sh own to reassemble into filament structures after denaturation and rena turation showing that HrpA alone is sufficient Far formation of filame nt structures. (C) 1997 Federation of European Biochemical Societies.