TUMOR-NECROSIS-FACTOR-ALPHA BINDING TO HUMAN AND MOUSE TROPHOBLAST

Tumour necrosis factor at (TNF-a) is a cytokine with pleiotropic effec ts, modulating cell growth, differentiation, and synthesis of various substances, Recent demonstration of TNF-alpha mRNA and protein in the uteroplacental unit suggests that this cytokine may be involved in the development of the embryo, To determine whether the embryo itself bin ds TNF-alpha, mouse blastocyst outgrowths and human first trimester vi llous trophoblast were analysed for TNP-alpha binding, Our experiments revealed that binding of TNF-alpha could be specifically detected on the trophectoderm of the outgrowing mouse embryos, They also show a co mplete disappearance of the colony-stimulating factor 1 (CSF-1) recept or that occurs shortly after the binding of TNF-alpha by the trophecto derm. In human first trimester villous trophoblast, TNF-alpha binding was found to be predominantly detectable on the syncytiotrophoblast an d to a lesser extent on the cytotrophoblastic cells, Binding was not o bserved on adjacent embryonic or maternal cells, Our results further s upport the idea that TNF-alpha as well as other cytokines may modulate early embryonic development and implantation. (C) 1997 Academic Press Limited.