HETEROLOGOUS EXPRESSION OF HUMAN DRUG-METABOLIZING-ENZYMES

This article is a report on a symposium held at the March 1997 meeting of the American Society for Pharmacology and Experimental Therapeutic s in San Diego. Current developments in the heterologous expression of cytochrome P450, NADPH-cytochrome P450 reductase, glutathione transfe rase, and UDP-glucuronosyltransferase enzymes are described, Systems i nclude bacteria, insect cells, and transient and stable mammalian cell s. Uses of the products are described for discernment of which enzymes are involved in metabolism of drugs, genotoxicity assays, mutagenesis (for structure-activity relationships), large scale production of enz yme products, antibody production, and production of proteins for biop hysical studies.