IDENTIFICATION OF TYPE-III SECRETED PRODUCTS OF THE PSEUDOMONAS-AERUGINOSA EXOENZYME-S REGULON

Extracellular protein profiles from wild-type and regulatory or secret ory isogenic mutants of the Pseudomonas aeruginosa exoenzyme S regulon were compared to identify proteins coordinately secreted with ExoS. D ata from amino-terminal sequence analysis of purified extracellular pr oteins were combined with data from nucleotide sequence analysis of lo ci linked to exoenzyme S production. We report the identification of P . aeruginosa homologs to proteins of Yersinia spp. that function as re gulators of the low calcium response, regulators of secretion, and med iators of the type III translocation mechanism.