STREPTOMYCES-LIVIDANS GROES, GROEL1 AND GROEL2 GENES

The Streptomyces lividans groES/EL1 operon and groEL2 gene were cloned and their respective DNA sequences determined. The sequenced DNA comp rised the genes and their respective regulatory regions in both cases. Transcription of both groES/EL1 and groEL2 seemed to be subjected to temporal control at 30 degrees C. At 45 degrees C the amount of the gr oEL2 transcript increased considerably in comparison to that of groES/ EL1. Among the proteins synthesized under heat shock by S. lividans, a fraction enriched in GroEL2 showed the presence of a ring-shaped stru cture that resembles that of other chaperonins and was active in a rho danase folding assay.