Dg. Ebo et al., LATEX-SPECIFIC IGE, SKIN TESTING, AND LYMPHOCYTE-TRANSFORMATION TO LATEX IN LATEX ALLERGY, Journal of allergy and clinical immunology, 100(5), 1997, pp. 618-623
Background: This study was designed to determine the discriminative va
lue of latex-specific IgE tests, latex skin tests, and lymphocyte tran
sformation tests (LTTs) to latex in 38 patients with latex allergy (12
nonatopic and 26 atopic) and 44 control subjects (24 nonatopic and 20
atopic). We also evaluated the recommended positive cutoff (i.e., 0.3
5 kU/L) of both in vitro latex-IgE tests. Methods: Latex-specific IgE
levels were determined by the Immuno-CAP (Upjohn-Pharmacia) and the AL
aSTAT-RIA (Diagnostic Products Corp.) assays, Skin tests and LTTs were
performed with a nonammoniated latex extract (DPC). Sensitivities and
specificities were defined according to the 95th percentile value of
nonatopic control subjects, For the in vitro IgE tests, sensitivity an
d specificity were also calculated by using the proposed positive thre
shold of 0.35 kU/L. Sensitivities and specificities of both cutoffs we
re compared. Results: Compared with a clinical history of latex allerg
y and according to the 95th percentile value of nonatopic control subj
ects (0.44 kU/L), latex-specific IgE determined by the Immuno-CAP assa
y achieved a sensitivity of 97% and a specificity of 86%. For the ALaS
TAT-RIA assay, with 0.54 kU/L as the 95th percentile threshold value i
n nonatopic control subjects, sensitivity was 100%, and specificity wa
s 83%. According to the threshold value of 0.35 kU/L, a sensitivity of
97% and a specificity of 83% for the Immuno-CAP assay and a sensitivi
ty of 100% and a specificity of 33% for the ALaSTAT-RIA assay were obs
erved. The latex skin test reached a sensitivity of 97% and a specific
ity of 100%. The LTT to latex showed a sensitivity of 39% and a specif
icity of 95%. No relation between symptoms and latex-specific IgE test
s, latex skin tests, or LTTs nas found. Conclusions: Our results confi
rm that latex skin tests and latex-specific IgE assessments are sensit
ive and specific methods for establishing the diagnosis of latex aller
gy, although the specificity of the ALaSTAT-RIA assay was very low whe
n interpreted according to the threshold of 0.35 kU/L. The LTT to nona
mmoniated latex is too insensitive for diagnosis of allergy to latex T
his reemphasizes that in order to evaluate the sensitivity and specifi
city of diagnostic procedures, one should always include an appropriat
e control group.