EFFECTS OF PRETREATMENT WITH AN INDENO-INDOLE COMPOUND ON LIPID-PEROXIDATION IN THE CORTEX AND MEDULLA OF RABBIT KIDNEYS AFTER ISCHEMIA-REPERFUSION

The effects of 60 min of ischaemia with or without reoxygenation in vi vo or in vitro on lipid peroxidation in cortical and medullary tissue from rabbit kidneys were measured as production of thiobarbituric acid -reactive substances (TBARS). Lipid peroxidation was more pronounced i n medullary tissue compared with cortical tissue. The highest TBARS pr oduction was found in medullary slices subjected to reoxygenation in v itro immediately after 1 h of ischaemia. Reperfusion in vivo before re oxygenation in vitro attenuated the TBARS formation during subsequent in vitro incubation. Pretreatment of the rabbits with an indeno-indole compound (code name H 290/51) reduced the TBARS formation after 60 mi n of ischaemia and reoxygenation in vitro towards control values.