IMMOBILIZATION OF LIPASE ON VARIOUS ACRYLIC COPOLYMERS

Polymer carriers with various degrees of hydrophobicity, porosity, and polarity were studied for immobilization of lipase from Candida rugos a. Copolymers and terpolymers were prepared by suspension polymerizati on of six monomers, ethyl and butyl acrylate, acrylonitrile, methyl me thacrylate, 2-hydroxypropyl methacrylate and vinyl acetate, and three crosslinking agents, divinylbenzene, ethylene glycol dimethacrylate an d trimethylolpropane triacrylate, in the presence of various diluents. Immobilization of lipase was carried out via glutaraldehyde chemical binding or by adsorption. It was found that the best results were obta ined for carriers of butyl acrylate, ethyl acrylate or 2-hydroxypropyl methacrylate crosslinked with ethylene glycol dimethacrylate. (C) 199 7 Elsevier Science S.A.