THE USE OF ETHIDIUM-BROMIDE TO ASSESS A NOVEL INJURY RECOVERY PHENOMENON IN LISTERIA-MONOCYTOGENES IN INHIBITORY NACL CONDITIONS/

Authors
ROBINSON TP OCIO MJ LYN F KALOTI A MACKEY BM
Citation
Tp. Robinson et al., THE USE OF ETHIDIUM-BROMIDE TO ASSESS A NOVEL INJURY RECOVERY PHENOMENON IN LISTERIA-MONOCYTOGENES IN INHIBITORY NACL CONDITIONS/, Letters in applied microbiology, 25(5), 1997, pp. 367-370
Citations number
24
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
25
Issue
5
Year of publication
1997
Pages
367 - 370
Database
ISI
SICI code
0266-8254(1997)25:5<367:TUOETA>2.0.ZU;2-E
Abstract
An injury and recovery phenomenon was observed in Listeria monocytogen es inoculated into a medium containing 2.2 mmol l(-1) NaCl, a concentr ation that was inhibitory to growth. The apparent loss then recovery o f viability, as determined by plate counts, was compared with the upta ke of ethidium bromide by the cells and found to be inversely related. Injury was caused not only by the initial osmotic up-shock but also b y the subsequent down-shock involved in the spread plate protocol.