W. Guido et al., DEVELOPMENTAL-CHANGES IN THE PATTERN OF NADPH-DIAPHORASE STAINING IN THE CATS LATERAL GENICULATE-NUCLEUS, Visual neuroscience, 14(6), 1997, pp. 1167-1173
We examined the pattern of NADPH-diaphorase (NADPH-d) staining in the
lateral geniculate nucleus (LGN) of dorsal thalamus in fetal and newbo
rn kittens, and adult cats. This staining Visualizes the synthesizing
enzyme of nitric oxide (NO), a neuromodulator associated with central
nervous system (CNS) development and synaptic plasticity. In the adult
, very few LGN cells stained for NADPH-d, and these were restricted to
interlaminar zones and ventral C layers. NADPH-d labeled a dense netw
ork of fibers and axon terminals throughout the LGN and adjacent thala
mic nuclei. The source of such labelling has been reported to be choli
nergic neurons from the parabrachial region of the brain stem (Bickfor
d et al., 1993). A very different pattern of staining was observed in
prenatal and early postnatal kittens. Between embryonic (E) day 46-57,
lightly stained cells appeared throughout the LGN. From this age, thr
ough about the first month of life, the number of stained cells in the
LGN rose rapidly. The density (cells/mm(2)) of labeled cells peaked a
t postnatal day (P) 28 (P28), and was about 150 times greater than the
level measured in the adult LGN. After P28, cell staining declined ra
pidly, and fell to adult levels at P41. The reduction in cell staining
that occurred between P35-41 was accompanied by the appearance of fin
e-caliber fiber staining, similar to that observed in the adult LGN. N
ADPH-d staining, which reveals the presence of nitric oxide synthase (
NOS), and thus NO activity, may reflect two processes. In the adult LG
N, the labeling of cholinergic axons arising from the brain-stem parab
rachial region coupled with a paucity of the LGN cellular staining sug
gests that NO operates in an orthograde manner, being co-released with
ACh to influence the gain and efficacy of retinogeniculate transmissi
on. By contrast, in developing kitten, NADPH-d staining of LGN cells s
uggests that NO acts in a retrograde fashion, perhaps playing a role i
n maintaining associative processes underlying activity-dependent refi
nement of retinogeniculate connections.