DETERMINATION OF FLUOXETINE IN HUMAN PLASMA USING REVERSED-PHASE HPLC

A rapid, simple, accurate method for the determination of fluoxetine i n human plasma is presented. Liquid-liquid extraction of fluoxetine wa s carried out using diethyl ether. Chlorprothixene was applied as an i nternal standard. The samples were chromatographed on a LiChrosorb RP- 18 (10 mu m) column and the mobile phase was acetonitrile/phosphate bu ffer pH 2.70 (9:1). The detection was carried at 254 nm. A linear quan titative response curve was generated over a concentration range of 10 0-600 ng/ml. Overall extraction efficiency of the extraction procedure was found to be 86 to 91% with a correlation coefficient of 0.992.