Pj. Fracica et al., NATURAL SURFACTANT AND HYPEROXIC LUNG INJURY IN PRIMATES .2. MORPHOMETRIC ANALYSES, Journal of applied physiology, 76(3), 1994, pp. 1002-1010
Natural surfactant and hyperoxic lung injury in primates. II. Morphome
tric analyses. J. Appl. Physiol. 76(3): 1002-1010, 1994. - Diffuse lun
g injury is accompanied by low compliance and hypoxemia with histologi
cal evidence of endothelial and alveolar epithelial cell disruption. T
he histological effects of treatment of an acute diffuse lung injury w
ith a natural surfactant product were evaluated in a primate model bec
ause surfactant function and content have been shown to be abnormal in
diffuse lung injury in both animals and humans. Ten baboons were vent
ilated with 100% O-2 for 96 h, and 5 were given an aerosol of natural
porcine surfactant. Physiological and biochemical measurements of the
effects of hyperoxia and surfactant treatment are presented in a compa
nion paper. After O-2 exposure, lungs were fixed and processed for qua
ntitative electron microscopy. The responses to O-2 included epithelia
l and endothelial cell injuries, interstitial edema, and inflammation.
The hyperoxic animals treated with surfactant were compared with the
untreated animals; the treatments altered neutrophil distribution, fib
roblast proliferation, and changes in the volumes of type I epithelial
cells and endothelial cells. Surfactant-treated animals also had decr
eased lamellar body volume density in type II epithelial cells and pre
servation of endothelial cell integrity. These changes suggest complex
effects of natural surfactant on the pulmonary response to hyperoxia,
including protection against epithelial and endothelial cell destruct
ion as well as significant interstitial inflammation and fibroblast pr
oliferation. We conclude that natural surfactant treatment of hyperoxi
c lung injury in primates resulted in partial protection of epithelial
and endothelial cells but also increased the accumulation of fibrobla
sts in the lung.