Lw. Li et al., A FAMILY OF PUTATIVE TUMOR SUPPRESSORS IS STRUCTURALLY AND FUNCTIONALLY CONSERVED IN HUMANS AND YEAST, The Journal of biological chemistry, 272(47), 1997, pp. 29403-29406
In Saccharomyces cerevisiae the CDC14 gene is essential for cell cycle
progression, Strains carrying the cdc14-1(ts) allele enter the cell c
ycle and arrest at restrictive temperatures, We have identified two hu
man cDNAs encoding proteins which share sequence identity to the yeast
CDC14p. The cell cycle arrest in cdc14-1(ts) can be specifically comp
lemented by the human cDNAs suggesting that they are functionally equi
valent to the yeast CDC14 gene, Another clone identified in the search
for human CDC14-like proteins corresponded to the putative tumor supp
ressor gene PTEN/MMAC1 (phosphatase and tensin homologue deleted on ch
romo same 10 or mutated in multiple advanced cancers 1), Analysis of t
he PTEN/MMAC1 showed that it did not complement the cdc14-1(ts) allele
and that it is more closely related to the yeast open reading frame Y
NL128W. Human CDC14p and PTEN/MMAC1 were expressed as recombinant prot
eins, and both were shown to have kinetic properties characteristic of
dual specific phosphatases, The human CDC14p was localized in the nuc
leus while PTEN/MMAC1 has been reported to be localized in the cytopla
sm, Our results suggest that CDC14 and YNL128W/PTEN/MMAC1 represent tw
o related, but distinct, families of human and yeast phosphatases.