NOVEL DIAZONIUM-FUNCTIONALIZED SUPPORT FOR IMMOBILIZATION EXPERIMENTS

A hydrophilic, water-insoluble polymer was prepared, starting from com mercial poly(vinyl alcohol) that was crosslinked and functionalized by means of glutaraldehyde and 4-nitrobenzaldehyde. The resulting beads were then reduced and subsequently diazotized, and finally contained d iazonium moieties capable of covalently coupling with electron-rich ar omatic systems such as histidine and/or tyrosine residues of proteins. The described resin is therefore well suitable for protein immobiliza tion whenever lysine residues (those involved in covalent coupling wit h several popular immobilization procedures) are not available and/or cannot be used unless the biological activity of the protein is destro yed. (C) 1997 John Wiley & Sons, Inc.