CELL BIOLOGISTS SORT THINGS OUT - ANALYSIS AND PURIFICATION OF INTRACELLULAR ORGANELLES BY FLOW-CYTOMETRY

Flow cytometry was established originally for measuring DNA content an d for the analysis of cell-surface markers in combination with cell so rting. During the past two decades, it has added new dimensions to var ious areas of immunology and medicine. Increased sensitivity and preci sion of flow cytometers, accompanied by the development of new fluores cent dyes and probes, has led to new applications in molecular cell bi ology and genetics. This article focuses on applications of flow cytom etry in analysis and sorting of intracellular organelles.