Mc. Sekar et al., DEMONSTRATION OF THE PRESENCE OF CYCLIC INOSITOL PHOSPHOHYDROLASE IN HUMAN URINE, Biochemical and molecular medicine, 62(1), 1997, pp. 95-100
Cyclic inositol phosphohydrolase (cIPH), cleaves the cyclic bond of cy
clic inositol monophosphate (cIP) to yield inositol monophosphate. In
this communication, we demonstrate the presence of cIPH in human urine
. cIPH was measured in the 24-h urine samples of both male and female
hospital patients. cIPH released per day ranged from 0 to 243 units in
men (n = 16) and from 15 to 346 units in women (n = 18). Release of c
IPH activity was not related to renal function as measured by creatini
ne clearance. HPLC ion-exchange chromatography or HPLC gel filtration
of ammonium sulfate precipitate yielded a distinct cIPH peak with an a
pparent molecular weight of 40 kDa on gel filtration. This is the firs
t demonstration of the presence of this enzyme in human urine. The lar
ge variation (over 20-fold) in the excretion of this protein suggests
that it may have physiological and/or pathological significance. (C) 1
997 Academic Press.